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2.6 Real-time PCR analysis Total RNA was prepared from liver tissues and LO2 cells using the simple RNA Extract kit (Tiangen Biotech, China) and reverse transcription of RNA to cDNA using Fast King cDNA First Strand Synthesis Kit (Tiangen Biotech, China)
Iron Supplement (Oral Route, Parenteral Route)
Contraindications or require Caution: individuals who are pregnant or breastfeeding, active malignancy (cancer), untreated hypothyroidism, intracranial lesions/tumors, and acute critical illness
doi: 10.1371/journal.pone.0048175 93 RohJLJangHKimEHShinD
Induction is problematic in drug development and an undesirable characteristic for a drug, thus drugs that have Phase II metabolism as their primary route of elimination may be easier to develop
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